Publications & Patent

Protein Engineering Lab



-Yea K, Jones TM, Jung D, Shin S, Arlian BM, Han KH, Zha Z, Kim MS, Oh YS, Zhang H, Lerner RA.An agonist antibody prefers relapsed AML for induction of cells that kill each other. Sci Rep. 2019 Mar 5;9(1):3494. doi: 10.1038/s41598-019-40087-7.



-Bucher F, Lee J, Shin S, Kim MS, Oh YS, Ha S, Zhang H, Yea K. Interleukin-5 suppresses Vascular Endothelial Growth

  Factor-induced angiogenesis through STAT5 signaling. Cytokine. 2018  Jun 16. S1043-4666(18)30270-9

-Bucher F, Fridlander M, Yea K. Antibody targeting TsPan12/b-catenin signaling in vasoproliferative retinopathy. Oncotarget. 2017 Dec 18;9(16):12538-12539


-Zha Z, Bucher F, Nejatfard A, Zheng T, Zhang H, Yea K* and Lerner RA*. Interferon-γ is a mastercheckpoint regulator of cytokine-induced differentiation. Proc. Natl. Acad. Sci. USA, Accepted. In press. 2017 Jul 17. (Corresponding author)
-Bucher F, Zhang D, Aguilar E, Sakimoto S, Aguilar S, Rosenfeld M, Zha Z, Zhang H, Friedlander M* and Yea K*. Antibody-mediated inhibition of Tspan12 ameliorates vasoproliferative retinopathy through suppression of β-catenin signaling. Circulation. 2017 Mar 29. (Corresponding author)


-Ahn B, Soundarapandian MM, Sessions H, Peddibhotla S, Roth GP, Li JL, Sugarman E, Koo A, Malany S, Wang M, Yea K,  Brooks J, Leone TC, Han X, Vega RB, Kelly DP. MondoA coordinately regulates skeletal myocyte lipid homeostasis and insulin signaling. J Clin Invest. 2016 Aug 8.
-Zhang H, Du M, Xie J, Liu X, Sun J, Wang W, Xin X, Possani LD, Yea K, Lerner RA. Autocrine-Based Selection of Drugs that Target Ion Channels from Combinatorial Venom Peptide Libraries. Angew Chem Int Ed Engl. 2016 May 20. 


-Zhang H, Sturchler E, Zhu J, Nieto A, Cistrone PA, Xie J, He L, Yea K, Jones T, Turn R, Di Stefano PS, Griffin PR, Dawson PE, McDonald PH, Lerner RA. Autocrine selection of a GLP-1R G-protein biased agonist with potent antidiabetic effects. Nat  Commun. 2015 Dec 1
-Yea K, Zhang H, Lerner RA. Activating pleiotropic receptors to kill cancer cells. Cell Cycle. 2015 Nov 20:0
-Lerner RA, Grover RK, Zhang H, Xie J, Han KH, Peng Y, Yea K. Antibodies from combinatorial libraries use functional receptor pleiotropism to regulate cell fates. Q Rev Biophys. 2015 Nov;48(4):389-94.
-Yea K, Zhang H, Xie J, Jones TM, Lin C, Francesconi W, Berton F, Fallahi M, Sauer K, and Lerner RA. Agonist antibody that induces human malignant cells to kill one another. Proc. Natl. Acad. Sci.USA, 2015 Nov 10
-Song P, Kwon Y, Yea K, Moon HY, Yoon JH, Ghim J, Hyun H, Kim D, Koh A, Berggren PO, Suh PG, Ryu SH. Apolipoprotein a1 increases mitochondrial biogenesis through AMP-activated protein kinase. Cell Signal. 2015 Sep;27(9):1873-81.
-Yea K, Xie J, Zhang H, ZhangW and Lerner RA. Selection of multiple agonist antibodies from intracellular combinatorial libraries reveals that cellular receptors are functionally pleiotropic. Curr Opin Chem Biol. 2015 Jan 23;26C:1-7.


-Kim D, Kim J, Yoon JH, Ghim J, Yea K, Song P, Park S, Lee A, Hong CP, Jang MS, Kwon Y, Park S, Jang MH, Berggren PO,  Suh PG, Ryu SH. CXCL12 secreted from adipose tissue recruits macrophages and induces insulin resistance in mice.  Diabetologia. 2014 Jul;57(7):1456-65
-Xie J, Yea K, Zhang H, Moldt B, He L, Zhu J, Lerner RA. Prevention of cell death by antibodies selected from intracellular combinatorial libraries. Chemistry & Biology. 2014 Feb 20;21(2):274-83


-Yea K, Zhang H, Xie J, Terri TM, Yang G, Song BD, Lerner RA. Converting stem cells to dendritic cells by agonist

  antibodies from unbiased morphogenic selections. Proc Natl Acad Sci U S A. 2013 Aug 26. 2013 Sep 10;110 (37):14966-71 
-Zhang H, Yea K, Xie J, Lerner RA. Selecting agonists from single cells infected with combinatorial antibody libraries. Chemistry & Biology. 2013 May 23;20(5):734-41
-Kim BJ, Lee YS, Lee SY, Park SY, Dieplinger H, Yea K, Lee SH, Koh JM, Kim GS. Afamin stimulates osteoclastogenesis and bone resorption via Gi-coupled receptor and Ca2+/calmodulin-dependent protein kinase (CaMK) pathways. J Endocrinol Invest. 2013 Nov;36(10):876-82
-Xie J, Zhang H, Yea K, Lerner RA. Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cells. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8099-104


-Kim BJ, Lee YS, Lee SY, Park SY, Dieplinger H, Ryu SH, Yea K, Choi S, Lee SH, Koh JM, Kim GS. Afamin secreted from nonresorbing osteoclasts acts as a chemokine for preosteoblasts via the Akts-ignaling pathway. Bone. 2012 Sep;51(3):431-  40
-Lim S, Jang HJ, Park EH, Kim JK, Kim JM, Kim EK, Yea K, Kim YH, Lee-Kwon W, Ryu SH, Suh PG. Wedelolactone inhibits  adipogenesis through the ERK pathway in human adipose tissue-derived mesenchymal stem cells. J Cell Biochem. 2012 Nov;113(11):3436-45
-Mitra R, Nogee DP, Zechner JF, Yea K, Gierasch CM, Kovacs A, Medeiros DM, Kelly DP, Duncan JG. The transcriptional  coactivators, PGC-1α and β, cooperate to maintain cardiac mitochondrial function during the early stages of insulin resistance. J Mol Cell Cardiol. 2012 Mar;52(3):701-10


-Yoon JH, Song P, Jang JH, Kim DK, Choi S, Kim J, Ghim J, Kim D, Park S, Lee H, Kwak D, Yea K, Hwang D, Suh PG, Ryu SH. Proteomic analysis of tumor necrosis factor-alpha (TNF-α)-induced L6 myotube secretome reveals novel TNF-α- dependent myokines in diabetic skeletal muscle. J Proteome Res. 2011 Dec 2;10(12):5315-25
-Lim S, Jang HJ, Kim JK, Kim JM, Park EH, Yang JH, Kim YH, Yea K, Ryu SH, Suh PG. Ochratoxin A inhibits adipogenesis through the ERK-PPAR-gamma pathway in human adipose tissue-derived mesenchymal stem cells. Stem Cells Dev. 2011  Mar;20(3):415-26



-Kim JH, Park JM, Yea K, Kim HW, Suh PG, Ryu SH. Phospholipase D1 mediates AMP-activated protein kinase signaling for  glucose uptake. PLoS One. 2010 Mar 9;5(3):e9600
-Choi S, Kim J, Yea K, Suh PG, Kim J, Ryu SH. Targeted label-free quantitative analysis of secretory proteins from  adipocytes in response to oxidative stress. Anal Biochem. 2010 Jun 15;401(2):196-202
-Jun DJ, Na KY, Kim W, Kwak D, Kwon EJ, Yoon JH, Yea K, Lee H, Kim J, Suh PG, Ryu SH, Kim KT. Melanocortins induce  interleukin 6 gene expression and secretion through melanocortin receptors 2 and 5 in 3T3-L1 adipocytes. J Mol Endocrinol. 2010 Apr;44(4):225-36
-Kong JS, Yoo SA, Kim HS, Kim HA, Yea K, Ryu SH, Chung YJ, Cho CS, Kim WU. Inhibition of synovial hyperplasia, rheumatoid T cell activation, and experimental arthritis in mice by sulforaphane, a naturally occurring isothiocyanate. Arthritis Rheum. 2010 Jan;62(1):159-70
-Kim J, Choi YS, Lim S, Yea K, Yoon JH, Jun DJ, Ha SH, Kim JW, Kim JH, Suh PG, Ryu SH, Lee TG. Comparative analysis of the secretory proteome of human adipose stromal vascular fraction cells during adipogenesis. Proteomics. 2010 Feb;10(3):394-405



-Yea K, Kim J, Kwon T, Kim JH, Lee BD, Lee HJ, Lee S, Lim SY, Lee T.G, Baek MC, Park HS, Park KS, Ohba M, Suh PG and Ryu SH. Lysophosphatidylcholine activates adipocyte glucose uptake and lowers blood glucose levels in murine models of Type 1 and 2 diabetes. J Biol Chem. 2009 Dec;284(49):33833-40 
-Choi S, Cho K, Kim J, Yea K, Park G, Lee J, Ryu SH, Kim J, Kim YH. Comparative proteome analysis using amine-reactive isobaric tagging reagents coupled with 2D LC/MS/MS in 3T3-L1 adipocytes following hypoxia or normoxia. Biochem Biophys Res Commun.2009 May 22;383(1):135-40
-Yea K, Kim J, Lim S, Kwon T, Park HS, Park KS, Suh PG and Ryu SH. Lysophosphatidylserine regulates blood glucose by enhancing glucose transport in myotubes and adipocytes. Biochem Biophys Res Commun. 2009 Jan 23;378(4):783-8
-Yoon JH, Yea K, Kim J, Suh PG and Ryu SH. Comparative proteomic analysis of the insulin-induced L6 myotube secretome. Proteomics. 2009 Jan;9(1):51-60


-Lee HY, Yea K, Kim J, Lee BD, Chae YC, Kim HS, Lee DW, Kim SH, Cho JH, Jin CJ, Koh DS, Park KS, Suh PG, and Ryu SH.Epidermal growth factor increases insulin secretion and lowers blood glucose in diabetic mice. J Cell Mol Med. 2008  Sep-Oct;12(5A):1593-604
-Lim S, Choi JW, Kim HS, Kim YH, Yea K, Heo K, Kim JH, Kim SH, Song M, Kim JI, Ryu SH and Suh PG. A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity. Life Sci. 2008 Mar 26;82(13-14):733-40
-Yea K, Kim J, Lim S, Park HS, Park KS, Suh PG and Ryu SH Lysophosphatidic acid regulates blood glucose by stimulating myotube and adipocyte glucose uptake. J Mol Med. 2008 Feb;86(2):211-20


- 사이토카인 융합 폴리펩티드, 및 이를 포함하는 사이토카인 라이브러리. PCT 국제출원. PCT/KR2019/007602. Issue date:2019.06.24

-멀티 채널 피펫 및 이를 이용한 조합 화학 분석 장치 및 방법. 특허번호 10-2019-0020051. Issue date : 2019/02/22.

-사이토카인 라이브러리 및 이를 이용한 생리활성 조절 인자의 스크리닝 방법. 대한민국. 특허번호 10-2018-0072220. Issue date : 2018/06/22.

​-항-Tspan12 항체 또는 그의 항원-결합 단편 및 그의 용도. 특허번호 10-2018-0036024. Issue date : 2018/03/28.

-Methods and compositions for producing activated Natural Killer cells and related uses.

 Richard A. Lerner, Kyungmoo Yea, Hongkai Zhang, Jia Xie. PCT, PATENT #:WO 16/205784, Issue date : 12/22/16